Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method

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The precautions are ancillary to the testing procedures and are not intended to be all inclusive.

AATCC grants you a license as follows: Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies. The criterion for passing the test must be agreed upon by the interested parties. Therefore, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method fulfills this need.

They may be any convenient size. At the conclusion of two weeks, make a fresh transplant from stock culture. This may be accomplished more easily by pressing the specimen to nethod agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use.

AATCC Test Method

Therefore, every necessary and 5. If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen.


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A standard strain of bacteria is used which is specific to the requirements of the material under test. Specimens of the test material, including corresponding untreated controls of the same material, are placed in intimate contact with growth agar which has been previously streaked with test organism.

AATCC Antibacterial Parallel Streak Test Method

Mix well using appropriate agitation. Antibacterial Activity Assessment of Textile Materials: Avoid mutation by strict adherence to aahcc stock transfers. All OSHA standards and rules must also be consulted and followed.

A zone of inhibition occurs as a result of the diffusion of an antimicrobial agent from the specimen. Rectangular specimens cut 25 – 50 mm They can be any convenient size.

The hard copy print may only be distributed to other employees for their internal mtehod within your organization. Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity. The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing residual amounts methld antibacterial agents as determined by chemical assay after multiple standard washings.


Take care not to break the surface of the agar while making the streaks.

AATCC 147 – Antimicrobial Fabric Test

Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide.

Notes and References Precision and Bias 7.

The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak to the next resulting in increasing degrees of sensitivity. Report of results will include an observation of zones of inhibition and growth under the specimen if present.

Wear safety glasses aatc all laboratory areas.

Your comments are invited either for revision of this test method or for additional methods and should be addressed to the AATCC Technical Center. Wear protective clothing and respiratory protection that prevents penetration by the bacteria. Mehod average width of a zone atacc inhibition along a streak on either side of the test specimen may be calculated using the following equation: In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise.