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Antibiotics analyzed included amikacin, ceftazidime, ciprofloxacin, cefepime, gentamicin, meropenem and piperacillin—tazobactum. FarrellRodrigo E. Telavancin is a lipoglycopeptide antibiotic with potent in vitro bactericidal activity when tested against Gram-positive bacteria, including methicillin-susceptible Staphylococcus aureus MSSAmethicillin-resistant S.

These changes were shown to improve drug solubility during panel preparation DMSO and drug availability in the well plastic plates Presulting in a lcsi accurate in vitro assessment of telavancin MIC determinations data on file; Theravance, Inc. Of the E. TABLE 3 MIC result variations and summary of essential agreement rates between dry-form broth microdilution formulation panel Sensititre and revised reference method for telavancin.

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In effect this has led to a slightly higher meropenem resistance of Gentamicin is rarely used as monotherapy in treating gram positive bacteria and as such this difference in MIC cut off is unlikely to be clinically significant. TABLE 1 MIC result variations and summary of essential agreement rates between previously established broth microdilution method and revised reference method for telavancin.

As for nitrofurantoin, its ability to concentrate in urine enables it to achieve significant concentrations and eliminate isolates that may have intermediate susceptibility.

Initial studies using this revised method observed that the MIC 50 results for telavancin were 4- to 8-fold lower than those obtained by the previous applied method use of DMSO and water as solvent and diluent for panel preparation, respectively, and no P supplementation when tested against staphylococci and enterococci, but minimal differences were observed when testing d23 data on s233 JMI Laboratories.


Subsequent investigations for oritavancin another lipoglycopeptide demonstrated that the addition of P to MIC testing broth was also necessary for test performance reliability via minimizing the drug binding to plastic well panels 5similar to dalbavancin. Background Over the last two decades, there has k100 emergence and spread cclsi antibiotic resistance in many bacterial clinical pathogens [ 1 ].

The results obtained are also limited to MICs generated by an clsii bacterial identification system which is not widely used in developing countries. Does the adoption of EUCAST susceptibility breakpoints affect the selection of antimicrobials to treat acute community-acquired respiratory tract infections? EUCAST eliminated the intermediate category for vancomycin in a bid to discourage the reporting of Glycopeptide-intermediate Staphylococcus aureus GISA due to poor response even to increased doses of vancomycin [ 816 flsi.

Lastly, the telavancin in vitro MIC results tested against Gram-positive organisms by the revised BMD method are now comparable to those reported for other lipoglycopeptide agents i.

This is another aspect that will require harmonization. The previous method produced most MIC results against S. Accessed 31 Aug Ann Clin Microbiol Antimicrob. The difference between the two susceptibility cut-offs is two dilutions and this may require further harmonization.

Results A total of E. Failure to stay updated may result in misinterpretation of susceptibility. The determination of accurate antibiotic susceptibility is therefore an important cog in the clinical care of bacterial infections especially in n100 that possess acquired resistance mechanisms and careful consideration should be given when deciding how to interpret phenotypic susceptibility data [ 11 ]. Our study is limited by the fact that we only compared the susceptibility for three bacteria whose results may not necessarily be generalizable to all clinically relevant gram positive and negative bacteria.

These results suggest that i P is necessary for a more accurate MIC determination for telavancin and previous studies underestimated the drug’s in vitro potency due to drug loss because of binding to plastic surfaces 1213— 15 and ii similar to dalbavancin and oritavancin, presence of LHB provides an effect similar to that of P With EUCAST guidelines being freely available it should be considered as an alternative especially in resource poor settings in order to maintain up-to-date antibiotic susceptibility interpretation.


Given that EUCAST guidelines are freely available, it makes it easier for laboratories in resource poor settings to have an updated and readily available reference for interpreting antibiotic susceptibilities. Some of the isolates included in this set 22 strains were provided by the Network on Antimicrobial Resistance in S. Secondly, details on the decision making process are not accessible to the public.

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The morbidity and mortality associated with communicable diseases including bacterial infections is quite significant in developing countries [ 10 ]. For the P.

In contrast, when tested against streptococci, the impact of the revised method on the telavancin MIC results was less pronounced, which was similar to those observed for the other lipoglycopeptides 45. Jones are employees of JMI Laboratories who receive grant funds to study telavancin and were paid consultants s32 Theravance in connection with the development of the manuscript.

TABLE 2 In vitro MIC results for telavancin when tested against Gram-positive isolates using previously established broth microdilution method and revised reference method. National Center for Biotechnology InformationU. This article has been cited by other articles in PMC. The revised method provided MIC results 2- to 8-fold lower than the previous method when tested against staphylococci and enterococci, resulting in MIC 50 values of 0.